Aseptic cryoprotectant-free vitrification of human ICSI spermatozoa
We would like to call your attention to the following One-on-One Training Course (hands-on workshop) on Aseptic cryoprotectant-free vitrification of human ICSI spermatozoa in Cologne and extend a cordial invitation to you. The course is an exclusive face-to-face individual training approach to facilitate learning that is anticipated to provide value-for-cost for participants because it is more effective than group training.
This workshop is intended for postgraduate students and specialists in the field of mammalian reproductive cells cryopreservation (in medicine and veterinary medicine).
At present, businesses that manufacture something new for clinical use find it much more difficult and time consuming than it was some years ago. We are announcing our workshop today because we are now able to present the plastic elements of the kits that had not previously been manufactured by firms and we had not the possibility of presenting it at our workshop.
Our course contains short basic lectures and a film regarding the technology. The central part of our workshop is hands-on manipulations.
During this workshop participants will obtain skills and kits (solutions, plastic and protocols) and will be ready when returning to their home laboratories to repeat the presented technology with respective results.
All demonstrating manipulations with human spermatozoa will be performed on real objects: fresh ejaculates.
The comparative cryopreservations will be offered for your review. For this comparison it will be possible during the workshop to use your home technology of sperm conventional (“slow”) freezing with permeable cryoprotectants. If you prefer to use your home freezing solutions for comparative cryopreservations, you can bring it (as well as instruments, plastic, containers etc.) to our workshop.
During the workshop we will assist you to perform the comparative investigations, we will separately and blind note obtained results, and we will compare our notations after the end of experimental cryopreservations. During these experiments we will formulate the following parameters of technology: optimal volume of vitrifying spermatozoa suspension, optimal time elapsing from the expelling of capillaries from liquid nitrogen to the plunging into the warming medium and optimal volume of the warming (culture) medium.
A few concise paragraphs on technologies and the workshop
- Each element of the technology is a result of our research that we have already published [1–23, 50-52].
- Technologies of cryoprotectant-free vitrification of human, mouse, canine, stallion, donkey, ram, porcine, fish and cat (http://blog.cincinnatizoo.org/2015/05/14/glass-glass-baby-birth-of-healthy-kittens-following-sperm-vitrification-for-artificial-insemination/) spermatozoa are now realities [1–52].
- Technology we have developed is original. After warming, vitrified spermatozoa are immediately ready for further use without any additional treatment (centrifugation for removal of cryoprotectants, separation in the gradient and others). Technology allows obtaining the following: vitrifying 0.01 ml suspension of spermatozoa, warming this 0.01 ml and the same 0.01 ml use for ICSI. After warming, spermatozoa are free from sperm plasma.
- Vitrification kit includes only one osmotically neutral solution without permeable cryoprotectants.
- We recommend our technology not only for its simplicity but also because our technology better preserves spermatozoa from ejaculates of bed quality, like asthenozoospermia and cryptozoospermia compared to traditional “slow” freezing with permeable cryoprotectants.
- Prof. Dr. Peter Mallmann, Director of University Maternal Hospital
- Dr. (SU) Evgenia Isachenko, Biological Director of IVF-Laboratory, Department of Obstetrics and Gynaecology, Cologne University Hospital
- Prof. Dr. Gohar Rahimi, Medical Director of IVF-Laboratory, Department of Obstetrics and Gynaecology, Cologne University Hospital
- Prof. Dr. Raul Sanchez, Director of Biomedical Center, La Frontera University, Temuco, Chile (during certain periods only)
- Dr. (SU) Vladimir Isachenko, Leader of Research Group for Reproductive Medicine, Department of Obstetrics and Gynaecology, Cologne University Hospital
Further Information and Program
Date: Beginning October 2019 (any date at the participant’s convenience)
Duration: 2 days
Number of participants: One, a maximum of two per session
Location: University Hospital of Cologne, Department of Gynaecology and Obstetrics
Kerpener Str. 34, 50931 Cologne, Germany
Language: English and German
Course fee: 1000 EUR for a single participant, 1500 EUR for two participants
Information and registration:
Dr. (SU) Vladimir Isachenko
Phone +49 221 478-7349
About the place
Cologne is a famous tourist city. Staying in Cologne allows easy access to the Netherlands, Belgium, Luxemburg and France. For example, a tour to Amsterdam with a 5 to 6 hour stay in the city with transport Cologne-Amsterdam-Cologne occupies a little bit more than one light-day and costs up to 25 EUR. A 4-day tour to Paris (3 days in this city) including transport und hotel costs from 90 to 180 EUR.
|08:00 - 08:45||Lecture |
Cryobiology as a field of knowledge and application in reproductive medicine and oncology.
|Dr. (SU) Vladimir Isachenko|
|09:00 - 09:45||Lecture |
Cryoprotectant-free vitrification of human spermatozoa: theory and practical application.
|Dr. (SU) Evgenia Isachenko|
|10:00 - 10:45||Film with comments |
Cryoprotectant-free vitrification of mammalian spermatozoa.
|Dr. (SU) Vladimir Isachenko|
|11:00 - 12:45 and |
13:40 - 16:00
|Hands-on ||Dr. (SU) Vladimir Isachenko; Dr. (SU) Evgenia Isachenko; Prof. Dr. Gohar Rahimi; Prof. Dr. Raul Sanchez|
|08:00 - 08:45||Lecture |
Integration of spermatozoa in oocytes: assisted technology for artificial activation and correction of position of the pronuclei.
|Prof. Dr. Peter Mallmann|
|09:00 - 12:50 and 13:40 - 16:00||Hands-on ||Dr. (SU) Vladimir Isachenko; Dr. (SU) Evgenia Isachenko; Prof. Dr. Gohar Rahimi; Prof. Dr. Raul Sanchez|
|16:00 - 17:00||Extensive discussions and repeated hands-on manipulations ||Prof. Dr. Peter Mallmann; Dr. (SU) Evgenia Isachenko; Dr. (SU) Vladimir Isachenko; Prof. Dr. Gohar Rahimi; Prof. Dr. Raul Sanchez|
|17:00 - 17:15||Presentation of certificates and departure|
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